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Recombinant Human IL-4RA/CD124 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1684

Validation Data Gallery

  • Purity of Recombinant Human IL-4RA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IL-4RA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human IL-4RA protein Met26-His232 (Accession# P24394-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 3566
Accession P24394-1
PredictedSize 50.0 kDa
SDS-PAGE 55-75 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production, as well as chemokine and mucus production at sites of allergic inflammation (PMID: 36440951; 23124025; 35934680).

References:

1. Drube S. et al. (2001). Immunology. 169(1):102-112.
2. Bhattacharjee A. et al. (2013). Free Radic Biol Med. 54:1-16.
3. Shankar A. et al. (2022). J Allergy Clin Immunol. 150(2):266-276.