AuthentiKine™ Human IL-10 ELISA Kit

Sensitivity

2.3 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Human

Cat no : KE00170

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF



Product Information

KE00170 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL10 ELISA kit is to be used to detect and quantify protein levels of endogenous IL10. The assay recognizes human IL10. An antibody specific for IL10 has been pre-coated onto the microwells. The IL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL10 is added to detect the captured IL10 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name AuthentiKine™ Human IL-10 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 2.3 pg/mL
Range 15.6-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
human plasma92%79%-100%
Cell culture supernatants102%88%-128%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12067.84.56.7
220175.010.05.7
320926.242.24.6

InterAssay

Samplenmean ( pg/mL)SDCV%
124193.917.69.1
224432.438.99.0
3241108.4111.310.0

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

Publications

SpeciesSample TypeTitle
humancell culture supernament

Cell Death Dis

Hypoxic glioma-derived exosomes promote M2-like macrophage polarization by enhancing autophagy induction.

Authors - Jianye Xu
humanserum

Front Immunol

Regulatory B Cells Dysregulated T Cell Function in an IL-35-Dependent Way in Patients With Chronic Hepatitis B.

Authors - YaYun Liu
humancell culture supernatants

Mol Ther

Exosomal miR-1246 from glioma patient body fluids drives the differentiation and activation of myeloid-derived suppressor cells.

Authors - Wei Qiu