AuthentiKine™ Human IL-10 ELISA Kit

Sensitivity

2.3 pg/ml

Range

15.6-1000 pg/mL

Reacted Species

Human

Cat no : KE00170

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF



Product Information

KE00170 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL10 ELISA kit is to be used to detect and quantify protein levels of endogenous IL10. The assay recognizes human IL10. An antibody specific for IL10 has been pre-coated onto the microwells. The IL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL10 is added to detect the captured IL10 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name AuthentiKine™ Human IL-10 ELISA Kit
Tests 1 X 96 well plate
Sample type plasma, serum, Cell culture supernatants
Assay type Sandwich
Sensitivity 2.3 pg/ml
Range 15.6-1000 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
human plasma92%79%-100%
Cell culture supernatants102%88%-128%

IntraAssay

Samplenmean ( pg/ml)SDCV%
12067.84.56.7
220175.010.05.7
320926.242.24.6

InterAssay

Samplenmean ( pg/ml)SDCV%
124193.917.69.1
224432.438.99.0
3241108.4111.310.0

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF