Recombinant Human IL-1R1 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3002

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Validation Data Gallery

• 

  Purity of Recombinant Human IL-1R1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human IL-1R1 protein Asp21-Thr332 (Accession# P14778) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 3554
• Accession: P14778
• PredictedSize: 61.9 kDa
• SDS-PAGE: 68-90 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin-1 Receptor Type 1 (IL1R1) is a key component of the immune system, particularly in the mediation of inflammatory responses. IL1R1 is the primary receptor through which the pro-inflammatory cytokine interleukin-1β (IL-1β) exerts its effects. It plays a central role in the development and regulation of both non-specific defense mechanisms and specific immunity. IL1R1 has three extracellular domains critical for cytokine binding and one intracellular domain responsible for signal transduction. The interaction with IL-1β leads to the activation of signaling pathways such as p38α-MAPK and NFκB, which are essential for inflammation and immune responses.

References:

1. J V Zhukova. et al. (2024). Cytokine Growth Factor Rev. 80:59-71.
2. Charles A Dinarello. et al.(2018). Immunol Rev. 281(1):8-27.
3. Lori Broderick. et al. (2022). Nat Rev Rheumatol. 18(8):448-463.
4. Theresa V Rohm. et al. (2022). Immunity. 55(1):31-55.