Recombinant Human IL-32 protein (isoform alpha, rFc Tag)
Species
Human
Purity
>85 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg3224
Validation Data Gallery
Product Information
| Purity | >85 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human IL-32 isoform alpha protein Met1-Lys131 (Accession# P24001-4) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 9235 |
| Accession | P24001-4 |
| PredictedSize | 41.2 kDa |
| SDS-PAGE | 29-31 kDa and 43-48 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Interleukin 32 (IL32) is a member of the cytokine family. IL32 contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. IL32 is a predominantly intracellular proinflammatory cytokine produced by epithelial cells, monocytes, T-lymphocytes and natural killer cells, and is involved in inflammation and cancer development. IL32 is able to induce the release a variety of pro-inflammatory cytokines such as IL8, tumor necrosis factor-α (TNFα) and IL6. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2.
References:
1.Dahl, C A et al. Journal of immunology (Baltimore, Md. : 1950) vol. 148,2 (1992): 597-603. 2.Kim, Soo-Hyun et al. Immunity vol. 22,1 (2005): 131-42. 3.Shoda, Hirofumi et al. Arthritis research & therapy vol. 8,6 (2006): R166.