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WB analysis of human saliva using 60099-1-Ig (same clone as 60099-1-PBS)
human saliva were subjected to SDS PAGE followed by western blot with 60099-1-Ig (Human IgA Antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.
WB analysis of human plasma using 60099-1-Ig (same clone as 60099-1-PBS)
human plasma were subjected to SDS PAGE followed by western blot with 60099-1-Ig (Human IgA Antibody) at dilution of 1:64000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:320000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:320000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 60099-1-Ig (same clone as 60099-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgA antibody (60099-1-Ig, Clone: 3A5A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60099-1-PBS in a different storage buffer formulation.

Human IgA Monoclonal antibody, PBS Only

Human IgA Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 60099-1-PBS

Clone No.3A5A5

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Human IgA Heavy Chain, IGHA1, 3A5A5, IgA, IgA1

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60099-1-PBS targets Human IgA in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag7417 Product name: Recombinant human IgA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 4-353 aa of BC016369 Sequence: TSPKVFPLSLCSTQPDGNVVIACLVQGFFPQEPLSVTWSESGQGVTARNFPPSQDASGDLYTTSSQLTLPATQCLAGKSVTCHVKHYTNPSQDVTVPCPVPSTPPTPSPSTPPTPSPSCCHPRLSLHRPALEDLLLGSEANLTCTLTGLRDASGVTFTWTPSSGKSAVQGPPERDLCGCYSVSSVLPGCAEPWNHGKTFTCTAAYPESKTPLTATLSKSGNTFRPEVHLLPPPSEELALNELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTTTFAVTSILRVAAEDWKKGDTFSCMVGHEALPLAFTQKTIDRLAGKPTHVNVSVVMAEVDGTCY Predict reactive species
Full Name	immunoglobulin heavy constant alpha 1
Calculated Molecular Weight	496 aa, 53 kDa
Observed Molecular Weight	55-60 kDa
GenBank Accession Number	BC016369
Gene Symbol	Human IgA Heavy Chain
Gene ID (NCBI)	3493
RRID	AB_1557448
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).