Human IgA Monoclonal antibody

Human IgA Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3A5A5

Cat no : 60099-1-Ig

Synonyms

IgA1, IGHA1



Tested Applications

Positive WB detected inhuman saliva tissue, human placenta tissue, human plasma tissue
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:25600-1:102400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60099-1-Ig targets Human IgA in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Human IgA fusion protein Ag7417
Full Name immunoglobulin heavy constant alpha 1
Calculated molecular weight 496 aa, 53 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC016369
Gene symbol IGHA1
Gene ID (NCBI) 3493
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).

Protocols

Product Specific Protocols
WB protocol for Human IgA antibody 60099-1-IgDownload protocol
IHC protocol for Human IgA antibody 60099-1-IgDownload protocol
IF protocol for Human IgA antibody 60099-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Nanobiotechnology

A milk-based self-assemble rotavirus VP6-ferritin nanoparticle vaccine elicited protection against the viral infection.

Authors - Zhipeng Li