Human IgA Monoclonal antibody
Human IgA Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
3A5A5
Cat no : 60099-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human saliva tissue, human saliva, human plasma |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human tonsillitis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:160000-1:640000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
60099-1-Ig targets Human IgA in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Human IgA fusion protein Ag7417 |
Full Name | immunoglobulin heavy constant alpha 1 |
Calculated molecular weight | 496 aa, 53 kDa |
Observed molecular weight | 55-60 kDa |
GenBank accession number | BC016369 |
Gene symbol | IGHA1 |
Gene ID (NCBI) | 3493 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Caprylic acid/ammonium sulfate precipitation |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Human IgA antibody 60099-1-Ig | Download protocol |
IHC protocol for Human IgA antibody 60099-1-Ig | Download protocol |
IF protocol for Human IgA antibody 60099-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Nanobiotechnology A milk-based self-assemble rotavirus VP6-ferritin nanoparticle vaccine elicited protection against the viral infection. |