Recombinant Human KIR2DL5/CD158f1 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg3489
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human KIR2DL5 protein His22-His238 (Accession# Q8N109) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 57292 |
| Accession | Q8N109 |
| PredictedSize | 49.6 kDa |
| SDS-PAGE | 55-70 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
KIR2DL5, also known as CD158f1, is a member of the human killer cell immunoglobulin-like receptor (KIR) family. KIR2DL5 has two extracellular Ig-like domains of the D0 and D2 type, a structural configuration that was previously unique to KIR2DL4. KIR2DL5 is expressed by subpopulations of NK cells and T cells. KIR2DL5 inhibits the activity of NK cells thus preventing cell lysis.
References:
1.Vilches C. et al. (2000). J Immunol. 164(11):5797-5804. 2.Cisneros E. et al. (2012). Front Immunol. 3:289. 3.Ren X. et al. (2022). J Clin Invest. 132(22):e163620.