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Recombinant Human MPIG6B protein (rFc Tag) (Eg5762)

Recombinant Human MPIG6B protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg5762

Overview

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Validation Data Gallery

Purity of Recombinant Human MPIG6B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Cat No. Eg5762

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Product Information

Purity	>90 %, SDS-PAGE
Endotoxin	<0.1 EU/μg protein, LAL method
Activity	Not tested
Expression	HEK293-derived Human MPIG6B protein Asn18-Gln142 (Accession# O95866-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID	80739
Accession	O95866-1
PredictedSize	39.0 kDa
SDS-PAGE	40-50 kDa, reducing (R) conditions
Formulation	Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution	Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions	It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Until expiry date, -20℃ to -80℃ as lyophilized proteins. 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

MPIG6B (megakaryocyte and platelet inhibitory receptor G6b), also known as G6b-B or C6orf25, is a lineage-specific immunoreceptor tyrosine-based inhibition motif (ITIM)-containing receptor belonging to the immunoglobulin superfamily. It is predominantly expressed on megakaryocytes and platelets, where it acts as a critical negative regulator of hematopoietic lineage differentiation, megakaryocyte maturation, and platelet production. G6b-B constitutively associates with the tyrosine phosphatases Shp1 and Shp2 to inhibit signaling from ITAM-containing receptors such as GPVI and CLEC-2, thereby attenuating platelet aggregation and activation. Loss-of-function mutations in MPIG6B in humans cause autosomal recessive congenital macrothrombocytopenia with focal myelofibrosis, characterized by severe thrombocytopenia, anemia, and bleeding diathesis.

References:

1. Mazharian, Alexandra et al. Science signaling vol. 5,248 ra78. 30 Oct. 2012, 2. Hofmann, Inga et al. Blood vol. 132,13 (2018): 1399-1412. 3. Newland, Stephen A et al. Blood vol. 109,11 (2007): 4806-9. 4. Becker, Isabelle C et al. Blood advances vol. 6,10 (2022): 3155-3161.