Recombinant Human MUC1/CA15-3 protein (hFc Tag)

• Home
• Products
• Fusion Proteins
• AuthentiKine ELISA Kits
• Recombinant Human MUC1/CA15-3 protein (hFc Tag) (Eg1039)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

hFc Tag

Activity

not tested

Cat no : Eg1039

---

Validation Data Gallery

• 

  Purity of Recombinant Human MUC1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

---

Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human MUC1 protein Ser24-Ser380 (Accession# P15941-1) with a human IgG1 Fc tag at the C-terminus.
• GeneID: 4582
• Accession: P15941-1
• PredictedSize: 61.2 kDa
• SDS-PAGE: 70-80 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

MUC1, also known as CA15-3, is a type I transmembrane glycoprotein expressed by various epithelial cells of the female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies.

References:

1. S J Gendler, et al. (1995) Annu Rev Physiol. 57:607-34.
2. Pankaj K Singh, et al. (2006) Trends Cell Biol. 16(9):467-76.
3. Ming-Kwang Shyu, et al. (2008) Biol Reprod. 79(2):233-9. 
4. Sritama Nath, et al. (2014) Trends Mol Med. 20(6):332-42.
5. Guang Wu, et al. (2018) Theranostics. 8(1):78-91.