Speedy™ Human NCAM1/CD56 One-Step ELISA Kit
Cat no : SE50088
Synonyms
CD56, NCAM1, MSK39, N CAM 1, NCAM
Validation Data Gallery
Product Information
| Product name | Speedy™ Human NCAM1/CD56 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Human cerebrospinal fluid (CSF) |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4684 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 101% | 95%-108% |
| CSF | 102% | 98%-108% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.20 | 0.07 | 1.35 |
| 2 | 8 | 1.31 | 0.04 | 3.05 |
| 3 | 8 | 0.64 | 0.02 | 3.13 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.21 | 0.13 | 2.50 |
| 2 | 16 | 1.30 | 0.03 | 2.31 |
| 3 | 16 | 0.64 | 0.02 | 3.13 |
Background Information
Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA. The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA). Several other isofroms have also been described.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD56, NCAM1, MSK39, N CAM 1, NCAM |