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Recombinant Human Neutrophil Elastase protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3192

Validation Data Gallery

  • Purity of Recombinant Human Neutrophil Elastase was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Neutrophil Elastase was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human Neutrophil Elastase protein Ser28-His267 (Accession# P08246) with a rabbit IgG Fc tag at the C-terminus.
GeneID 1991
Accession P08246
PredictedSize 52.1 kDa
SDS-PAGE 48-60 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation. Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity, which is reported to cause mucin secretion (degranulation). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration. The full length protein has a signal peptide,propeptide and three glycosylation sites.

References:

1.Kawabata K, Hagio T,Matsuoka S. (2002) Eur J Pharmacol. 451 (1):1-10.
2.Kohri K,Ueki IF,Nadel JA. (2002) Am J Physiol Lung Cell Mol Physiol. 283 (3):L531-40.
3.Carden, D et al.(1998) The American journal of physiology. 275, 2: H385-92.