Recombinant Human PMCH protein (rFc Tag)

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• Recombinant Human PMCH protein (rFc Tag) (Eg2978)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2978

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Validation Data Gallery

• 

  Purity of Recombinant Human PMCH was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human PMCH protein Ile22-Val165 (Accession# P20382) a rabbit IgG Fc tag at the C-terminus.
• GeneID: 5367
• Accession: P20382
• PredictedSize: 42.4 kDa
• SDS-PAGE: 44-50 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The orexigenic neuropeptide melanin-concentrating hormone (MCH), a product of PMCH, is an important mediator of energy homeostasis. PMCH is expressed predominantly in neurons of the lateral hypothalamic area and the incerto hypothalamic area, which project throughout the brain. PMCH is also expressed in some peripheral tissues, such as the testes, although at lower levels than in the brain. PMCH plays an important role in determining energy balance during early development.

References:

1.Mul JD. et al. (2010) Am J Physiol Endocrinol Metab. 298(3):E477-E488.
2.Mul JD. et al. (2013) PLoS One. 8(3):e60214.
3.Bertolesi GE. et al. (2019) Pigment Cell Melanoma Res. 32(4):510-527.