Recombinant Human ROBO4 protein (rFc Tag)

• Home
• Products
• Fusion Proteins
• AuthentiKine ELISA Kits
• Recombinant Human ROBO4 protein (rFc Tag) (Eg7821)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg7821

---

Validation Data Gallery

• 

  Purity of Recombinant Human ROBO4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

---

Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human ROBO4 protein Gln28-Arg467 (Accession# Q8WZ75-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 54538
• Accession: Q8WZ75-1
• PredictedSize: 73.1 kDa
• SDS-PAGE: 75-100 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

ROBO4 belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. ROBO4 may mediate the inhibition of primary endothelial cell migration by Slit proteins. It’s reported that activating Robo4 may have broad therapeutic application in diseases characterized by excessive angiogenesis and/or vascular leak.

References:

1. Jones, Christopher A et al. Nature medicine vol. 14,4 (2008): 448-53. 
2. Dai, Chang et al.Bioscience reports vol. 39,7 (2019): null.
3.Xiao W,Pinilla-Baquero A,Faulkner J, et al.Sci Rep. 2022;12 (1):4352.