Recombinant Human SMN protein (rFc Tag)

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• Recombinant Human SMN protein (rFc Tag) (Eg4812)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg4812

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Validation Data Gallery

• 

  Purity of Recombinant Human SMN was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human SMN protein Gln90-Asn151 (Accession# Q16637) with a rabbit IgG Fc tag at the C-terminus
• GeneID: 6607
• Accession: Q16637
• PredictedSize: 33.2 kDa
• SDS-PAGE: 34-44 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Survival Motor Neuron (SMN) protein required for efficient assembly of small nuclear ribonucleoprotein (snRNP) complexes is encoded by nearly identical telomeric and centromeric forms of SMN gene respectively. Both the SMN1 and SMN2 genes express SMN protein, however, the amount of functional full-length protein produced by SMN2 is much less than that produced by SMN1 due to the alternative splicing. The absence of SMN1 can be partially compensated for by SMN2 and the SMN2 expression level is associated with SMA severity.

References:

1. Lefebvre S, et al. (1995). Cell. 80(1):155-65.
2. Gavrilov DK, et al. (1998). Nat Genet. 20(3):230-1.
3. Monani UR, et al. (2000). Hum Mol Genet. 9(3):333-9.
4. Burghes AH, et al. (2009). Nat Rev Neurosci.10(8):597-609.