• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant Human SPINT2/HAI-2 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3130

Validation Data Gallery

  • Purity of Recombinant Human SPINT2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human SPINT2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human SPINT2 protein Ala28-Lys197 (Accession# O43291-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 10653
Accession O43291-1
PredictedSize 45.2 kDa
SDS-PAGE 48-60 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

SPINT2 (serine peptidase inhibitor Kunitz type 2), also known as hepatocyte growth factor activator inhibitor type-2(10), belongs to the Kunitz family of serine protease inhibitors, which is a plasma membrane-localized serine protease inhibitor found in epithelial cells of various tissues including the respiratory tract and all major organs. SPINT2 possesses one transmembrane domain and two kunitz-type inhibitor domains that are exposed to the extracellular space and which are believed to facilitate a potent inhibition of target proteases. SPINT2 regulates cell proliferation, migration and invasion via a reduction in HGFA, leading to apoptosis and necrosis of uterine leiomyosarcoma cells.

References:

1. Li J, et al. (2023). Exp Ther Med. Oct 9;26(6):546. 
2. Straus, M.R., et al. (2020). Virology. Apr;543:43-53. 
3. Heinz-Erian P, et al. (2009). Am J Hum Genet. 84(2):188-196.
4. Pereira, M.S., et al. (2016). J Histochem Cytochem. Jan;64(1):32-41.