Recombinant Human TIM-3 protein (rFc Tag)

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• Recombinant Human TIM-3 protein (rFc Tag) (Eg3357)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3357

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Validation Data Gallery

• 

  Purity of Recombinant Human TIM-3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human TIM-3 protein Ser22-Arg200 (Accession# Q8TDQ0-1) with a rabbit IgG Fc tag at the C-terminus
• GeneID: 84868
• Accession: Q8TDQ0-1
• PredictedSize: 46.1 kDa
• SDS-PAGE: 52-72 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection. TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity. TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3.

References:

1. Isabel Sada-Ovalle. et al.(2012) Nat Immunol.6(12):1245-52.
2. Chen Zhu. et al.(2005) Nat Immunol. 6(12):1245-52.
3. Mi Jin Lee. et al.(2009) Immune Netw.9(2):58-63.
4. Philipp S van de Weyer. et al.(2006) Biochem Biophys Res Commun. 351(2):571-6.
5. J J McIntire. et al.(2001) Nat Immunol. 2(12):1109-16.