Recombinant Human TMEM119 protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0796

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Validation Data Gallery

• 

  Purity of Recombinant Human TMEM119 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human TMEM119 protein Thr118-Val283 (Accession# Q4V9L6) with a His tag at the C-terminus.
• GeneID: 338773
• Accession: Q4V9L6
• PredictedSize: 18.4 kDa
• SDS-PAGE: 28-32 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

TMEM119, also known as Osteoblast Induction Factor (OBIF), plays important role in bone formation and normal bone mineralization. It is a highly expressed microglia-specific marker in both mouse and human. TMEM119 implicated in various cancers including gastric, osteosarcoma and hepatocellular carcinoma.

References:

1.Zheng P. et al. (2018) Oncol Lett. 15(6):8281-8286. 
2.Jiang ZH. et al. (2017) Exp Mol Med. 49(5):e329. 
3.Bai KH. et al. (2020) Cancer Med. 9(12):4290-4298.