AuthentiKine™ Human TNF-alpha ELISA Kit

Sensitivity

8.6 pg/mL

Range

15.6-500 pg/mL

Reacted Species

Human

Cat no : KE00154

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor



Product Information

KE00154 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNF-alpha ELISA kit is to be used to detect and quantify protein levels of endogenous TNF-alpha. The assay recognizes human TNF-alpha. An antibody specific for TNF-alpha has been pre-coated onto the microwells. The TNF-alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for TNF-alpha is added to detect the captured TNF-alpha protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name AuthentiKine™ Human TNF-alpha ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 8.6 pg/mL
Range 15.6-500 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human Plasma10282-117
Cell culture supernatants9585-106

IntraAssay

Samplenmean ( pg/mL)SDCV%
12052.52.03.8
22087.32.83.2
320184.85.83.1

InterAssay

Samplenmean ( pg/mL)SDCV%
12451.32.44.6
22486.22.12.5
324184.25.12.7

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression and inflammatory bowel disease (IBD).

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor

Publications

SpeciesTitle
human

Front Oncol

Senolytics (DQ) Mitigates Radiation Ulcers by Removing Senescent Cells.

Authors - Huilan Wang
human

Nanotoxicology

Role of inflammation in the malignant transformation of pleural mesothelial cells induced by multi-walled carbon nanotubes.

Authors - Xiaopei Huang