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Recombinant Human VEGFD protein (Myc Tag, His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

not tested

Cat no : Eg0184

Validation Data Gallery

  • Purity of Recombinant Human VEGFD was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human VEGFD was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human VEGFD protein Phe93-Ser201 (Accession# O43915) with a Myc tag and a His tag at the C-terminus.
GeneID 2277
Accession O43915
PredictedSize 17.2 kDa
SDS-PAGE 21-31 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Vascular endothelial growth factor-D (VEGFD) is a secreted glycoprotein that can activate VEGF receptors on the endothelium, is a mitogen for endothelial cells and promotes the growth and remodeling of blood vessels and lymphatic vessels. VEGFD undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. VEGFD has been established to be over-expressed in both tumor tissues and patients' serum samples in several types of human cancer.

References:

1. S A Stacker. et al. (2001) Nat Med. 7(2):186-91.
2. M G Achen. et al. (1998) Proc Natl Acad Sci U S A. 20;95(2):548-53.
3. Natalia Davydova. et al. (2016) J Biol Chem. 291(53):27265-27278.
4. Y Yamada. et al. (1997) Genomics. 42(3):483-8.