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Recombinant Human B7-H4 protein (Myc Tag, His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

not tested

Cat no : Eg0098

Validation Data Gallery

  • Purity of Recombinant Human B7-H4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human B7-H4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human B7-H4 protein Phe29-Ala258 (Accession# Q7Z7D3-1) with a Myc tag, His tag at the C-terminus.
GeneID 79679
Accession Q7Z7D3-1
PredictedSize 30.3 kDa
SDS-PAGE 40-70 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

B7‑H4, also named VTCN1, B7X, or B7S1, is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. B7-H4 is up-regulated on the surface of cancer cells and immunosuppressive tumor-associated macrophages (TAMs) in a variety of human cancers.

References:

1. Wang JY, et al. (2020). Cell Immunol. 347:104008.
2. Yu J, et al. (2024). Cell. 187 (17): 4713-4732.e19. 
3. Smith JB, et al. (2014). Gynecol Oncol. 34(1):181-189. 
4. Tringler B, et al. (2006). Gynecol Oncol.100(1):44-52.
5. Daha N A, et al. (2012). Ann Rheum Dis. 71(4): 567–571.