Tested Applications
| Positive WB detected in | HeLa cells, mouse lung tissue, mouse brain tissue, Jurkat cells |
| Positive IP detected in | HeLa cells, Jurkat cells |
| Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17170-1-AP targets IARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10696 Product name: Recombinant human IARS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 425-773 aa of BC010218 Sequence: KMSKSLGNVIHPDVVVNGGQDQSKEPPYGADVLRWWVADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADFNPETDSIPVNDMYVIDQYMLHLLQDLANKITELYKQYDFGKVVRLLRTFYTRELSNFYFSIIKDRLYCEKENDPKRRSCQTALVEILDVIVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVITVIEPGLLFEIIEMLQSEETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREESSYKVIVMPTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK Predict reactive species |
| Full Name | isoleucyl-tRNA synthetase 2, mitochondrial |
| Calculated Molecular Weight | 1012 aa, 114 kDa |
| Observed Molecular Weight | 114 kDa |
| GenBank Accession Number | BC010218 |
| Gene Symbol | IARS2 |
| Gene ID (NCBI) | 55699 |
| RRID | AB_2264448 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NSE4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IARS2 antibody 17170-1-AP | Download protocol |
| IHC protocol for IARS2 antibody 17170-1-AP | Download protocol |
| IP protocol for IARS2 antibody 17170-1-AP | Download protocol |
| WB protocol for IARS2 antibody 17170-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody targeting IARS2 (catalog no. 17170-1-AP) has 9 total citations. Citations appear in Nature Communications, Molecular Cell, Scientific Reports, Orphanet Journal of Rare Disease, Frontiers in Oncology, Frontiers in Genetics, Discovery Oncology, Physiological Reports, and Revista da Associação Médica Brasileira. Research fields span mitochondrial tRNA synthetase biology, mTORC1 signaling, immune modulation, cancer (lung and cervical), rare genetic diseases (Leigh syndrome), and endothelial cell function.
| Species | Application | Title |
|---|---|---|
Nat Commun Targeted degradation of extracellular mitochondrial aspartyl-tRNA synthetase modulates immune responses | ||
Mol Cell Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation. | ||
Sci Rep Mechanism of isoleucyl-tRNA synthetase 2 regulating proliferation and apoptosis of cervical cancer cells.
| ||
Orphanet J Rare Dis IARS2 mutations lead to Leigh syndrome with a combined oxidative phosphorylation deficiency | ||
Front Oncol The Oncogene IARS2 Promotes Non-small Cell Lung Cancer Tumorigenesis by Activating the AKT/MTOR Pathway.
| ||
Front Genet Single-Cell Transcriptional Profiling Reveals Sex and Age Diversity of Gene Expression in Mouse Endothelial Cells. |
Reviews
What customers say
The single review for this antibody is highly positive. A researcher using it for Western Blot on fibroblast samples at a 1:1000 dilution (lot 00061450) gave it a 5-star rating, noting that the antibody works well and produces a strong signal. Overall, the feedback indicates reliable performance with clear detection in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vera (Verified Customer) (07-14-2026) | Antibody works well, shows a strong signal.
|
