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IARS2 Polyclonal antibody

IARS2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 17170-1-AP


IARS2, IleRS, Isoleucine tRNA ligase

Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, mouse brain tissue, Jurkat cells
Positive IP detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17170-1-AP targets IARS2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IARS2 fusion protein Ag10696
Full Name isoleucyl-tRNA synthetase 2, mitochondrial
Calculated Molecular Weight 1012 aa, 114 kDa
Observed Molecular Weight 114 kDa
GenBank Accession NumberBC010218
Gene Symbol IARS2
Gene ID (NCBI) 55699
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.


Product Specific Protocols
WB protocol for IARS2 antibody 17170-1-APDownload protocol
IHC protocol for IARS2 antibody 17170-1-APDownload protocol
IF protocol for IARS2 antibody 17170-1-APDownload protocol
IP protocol for IARS2 antibody 17170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


humanIF, WB

Mol Cell

Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.

Authors - Sung-Hoon Kim

Discov Oncol

High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer

Authors - Haiqing Wang

Rev Assoc Med Bras (1992)

IARS2 regulates proliferation, migration, and angiogenesis of human umbilical vein endothelial cells.

Authors - Yue-Ming Yu

Front Oncol

The Oncogene IARS2 Promotes Non-small Cell Lung Cancer Tumorigenesis by Activating the AKT/MTOR Pathway.

Authors - Xin Di
  • KD Validated