Validation Data Gallery
|Positive WB detected in||HeLa cells, mouse lung tissue, mouse brain tissue, Jurkat cells|
|Positive IP detected in||HeLa cells, Jurkat cells|
|Positive IHC detected in||human liver cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17170-1-AP targets IARS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IARS2 fusion protein Ag10696|
|Full Name||isoleucyl-tRNA synthetase 2, mitochondrial|
|Calculated molecular weight||1012 aa, 114 kDa|
|Observed molecular weight||114 kDa|
|GenBank accession number||BC010218|
|Gene ID (NCBI)||55699|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.
|Product Specific Protocols|
|WB protocol for IARS2 antibody 17170-1-AP||Download protocol|
|IHC protocol for IARS2 antibody 17170-1-AP||Download protocol|
|IF protocol for IARS2 antibody 17170-1-AP||Download protocol|
|IP protocol for IARS2 antibody 17170-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The Oncogene IARS2 Promotes Non-small Cell Lung Cancer Tumorigenesis by Activating the AKT/MTOR Pathway.
Rev Assoc Med Bras (1992)
IARS2 regulates proliferation, migration, and angiogenesis of human umbilical vein endothelial cells.
Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.