Validation Data Gallery
|Positive WB detected in||HeLa cells, mouse lung tissue, mouse brain tissue, Jurkat cells|
|Positive IP detected in||HeLa cells, Jurkat cells|
|Positive IHC detected in||human liver cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17170-1-AP targets IARS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IARS2 fusion protein Ag10696|
|Full Name||isoleucyl-tRNA synthetase 2, mitochondrial|
|Calculated molecular weight||1012 aa, 114 kDa|
|Observed molecular weight||114 kDa|
|GenBank accession number||BC010218|
|Gene ID (NCBI)||55699|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.
|Product Specific Protocols|
|WB protocol for IARS2 antibody 17170-1-AP||Download protocol|
|IHC protocol for IARS2 antibody 17170-1-AP||Download protocol|
|IF protocol for IARS2 antibody 17170-1-AP||Download protocol|
|IP protocol for IARS2 antibody 17170-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.
High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer
Rev Assoc Med Bras (1992)
IARS2 regulates proliferation, migration, and angiogenesis of human umbilical vein endothelial cells.
The Oncogene IARS2 Promotes Non-small Cell Lung Cancer Tumorigenesis by Activating the AKT/MTOR Pathway.