IARS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

IARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 17170-1-AP

Synonyms

IARS2, IleRS, Isoleucine tRNA ligase

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Validation Data Gallery

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  WB analysis using 17170-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 17170-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17170-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17170-1-AP

  IP result of anti-IARS2 (IP:17170-1-AP, 4ug; Detection:17170-1-AP 1:800) with HeLa cells lysate 4000ug.

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  IP experiment of Jurkat using 17170-1-AP

  IP result of anti-IARS2 (IP:17170-1-AP, 4ug; Detection:17170-1-AP 1:800) with Jurkat cells lysate 4000ug.

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  IHC staining of human liver cancer using 17170-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17170-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17170-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17170-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17170-1-AP

  Immunofluorescent analysis of HepG2 cells, using IARS2 antibody 17170-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue, mouse brain tissue, Jurkat cells
• Positive IP detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Product Information

17170-1-AP targets IARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IARS2 fusion protein Ag10696
• Full Name: isoleucyl-tRNA synthetase 2, mitochondrial
• Calculated Molecular Weight: 1012 aa, 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: BC010218
• Gene Symbol: IARS2
• Gene ID (NCBI): 55699
• RRID: AB_2264448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.

Publications

Species	Application	Title
human	IF, WB	Mol Cell; Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.; Authors - Sung-Hoon Kim
human	WB	Discov Oncol; High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer; Authors - Haiqing Wang
human	WB	Rev Assoc Med Bras (1992); IARS2 regulates proliferation, migration, and angiogenesis of human umbilical vein endothelial cells.; Authors - Yue-Ming Yu
human	WB	Nat Commun; Targeted degradation of extracellular mitochondrial aspartyl-tRNA synthetase modulates immune responses; Authors - Benjamin S Johnson
human	WB	Orphanet J Rare Dis; IARS2 mutations lead to Leigh syndrome with a combined oxidative phosphorylation deficiency; Authors - Qiyu Dong
human	WB	Front Oncol; The Oncogene IARS2 Promotes Non-small Cell Lung Cancer Tumorigenesis by Activating the AKT/MTOR Pathway.; Authors - Xin Di ; KD Validated