IARS2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84764-5-RR (same clone as 84764-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84764-5-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84764-5-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocellular cancer using 84764-5-RR (same clone as 84764-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84764-5-RR (IARS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84764-5-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocellular cancer using 84764-5-RR (same clone as 84764-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84764-5-RR (IARS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84764-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84764-5-RR (same clone as 84764-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84764-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84764-5-RR (same clone as 84764-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84764-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84764-5-RR (same clone as 84764-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84764-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84764-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84764-5-RR against Human IARS2 were performed. The affinity constant is 0.154 nM.

IARS2 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84764-5-PBS

Clone No.242219G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242219G4, EC:6.1.1.5, IleRS, Isoleucine tRNA ligase, Isoleucine--tRNA ligase, mitochondrial

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Product Information

84764-5-PBS targets IARS2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10696

  Product name: Recombinant human IARS2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 425-773 aa of BC010218

  Sequence: KMSKSLGNVIHPDVVVNGGQDQSKEPPYGADVLRWWVADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADFNPETDSIPVNDMYVIDQYMLHLLQDLANKITELYKQYDFGKVVRLLRTFYTRELSNFYFSIIKDRLYCEKENDPKRRSCQTALVEILDVIVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVITVIEPGLLFEIIEMLQSEETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREESSYKVIVMPTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK

• Full Name: isoleucyl-tRNA synthetase 2, mitochondrial
• Calculated Molecular Weight: 1012 aa, 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: BC010218
• Gene Symbol: IARS2
• Gene ID (NCBI): 55699
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NSE4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.