IBTK Recombinant monoclonal antibody, PBS Only

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• IBTK Recombinant monoclonal antibody, PBS Only (84706-4-PBS)

• 

  WB analysis using 84706-4-RR (same clone as 84706-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84706-4-RR (IBTK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84706-4-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 84706-4-RR (same clone as 84706-4-PBS)

  WB result of IBTK antibody (84706-4-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-IBTK transfected A549 cells. This data was developed using the same antibody clone with 84706-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 84706-4-RR (same clone as 84706-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84706-4-RR (IBTK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84706-4-PBS in a different storage buffer formulation.

• Featured Product

IBTK Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84706-4-PBS

Clone No.242177D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

242177D1, BTBD26, BTKI, KIAA1417, RP1 93K22.1

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Product Information

84706-4-PBS targets IBTK in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag35421

  Product name: Recombinant human IBTK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 850-1000 aa of BC143745

  Sequence: VADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYSAKQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSEFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDIFLKEEINMEQNHSETMFKKAKTKAKKKPRKRSDSSGGYNLSD

• Full Name: inhibitor of Bruton agammaglobulinemia tyrosine kinase
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC143745
• Gene Symbol: IBTK
• Gene ID (NCBI): 25998
• RRID: AB_3743668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9P2D0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IBTK, also known as BTKI and KIAA1417, which is involved in stress response and tumor growth. It has 3 isoforms and has been found to be hyper-expressed in chronic lymphocytic leukemia and correlated with disease progression (PMID: 29317636).