IBTK Recombinant monoclonal antibody

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• IBTK Recombinant monoclonal antibody (84706-4-RR)

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  WB analysis of A549 using 84706-4-RR

  WB result of IBTK antibody (84706-4-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-IBTK transfected A549 cells.

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  WB analysis using 84706-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84706-4-RR (IBTK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 84706-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84706-4-RR (IBTK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

IBTK Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 84706-4-RR

Clone No.242177D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

242177D1, BTBD26, BTKI, KIAA1417, RP1 93K22.1

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, Jurkat cells, Ramos cells
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84706-4-RR targets IBTK in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag35421

  Product name: Recombinant human IBTK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 850-1000 aa of BC143745

  Sequence: VADQLLITRLKEICEVALTEKLTLKNAAMLLEFAAMYSAKQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSEFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDIFLKEEINMEQNHSETMFKKAKTKAKKKPRKRSDSSGGYNLSD

• Full Name: inhibitor of Bruton agammaglobulinemia tyrosine kinase
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC143745
• Gene Symbol: IBTK
• Gene ID (NCBI): 25998
• RRID: AB_3743668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9P2D0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IBTK, also known as BTKI and KIAA1417, which is involved in stress response and tumor growth. It has 3 isoforms and has been found to be hyper-expressed in chronic lymphocytic leukemia and correlated with disease progression (PMID: 29317636).