IBTK Recombinant antibody

IBTK {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA
Cat No. 84706-4-RR
Clone No.242177D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

RP1 93K22.1, KIAA1417, BTKI, BTBD26, 242177D1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells, Jurkat cells, Ramos cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84706-4-RR targets IBTK in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IBTK fusion protein Ag35421 Predict reactive species
Full Name inhibitor of Bruton agammaglobulinemia tyrosine kinase
Observed Molecular Weight160 kDa
GenBank Accession NumberBC143745
Gene Symbol IBTK
Gene ID (NCBI) 25998
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9P2D0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IBTK, also known as BTKI and KIAA1417, which is involved in stress response and tumor growth. It has 3 isoforms and has been found to be hyper-expressed in chronic lymphocytic leukemia and correlated with disease progression (PMID: 29317636).

Protocols

Product Specific Protocols
WB protocol for IBTK antibody 84706-4-RRDownload protocol
IHC protocol for IBTK antibody 84706-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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