ICAM-1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ICAM-1 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F9A8

Cat no : 60299-1-PBS

Synonyms

BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58

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Validation Data Gallery

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  WB analysis using 60299-1-Ig (same clone as 60299-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 60299-1-Ig (same clone as 60299-1-PBS)

  WB result of ICAM1 antibody (60299-1-Ig, 1:50,000) with si-Control and si-ICAM1 transfected Raji cells. . This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60299-1-Ig (same clone as 60299-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 60299-1-Ig (same clone as 60299-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 60299-1-Ig (same clone as 60299-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 60299-1-Ig (same clone as 60299-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60299-1-Ig (same clone as 60299-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60299-1-Ig (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60299-1-Ig (same clone as 60299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60299-1-Ig (same clone as 60299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60299-1-Ig (same clone as 60299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60299-1-Ig (same clone as 60299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60299-1-Ig (ICAM-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 60299-1-Ig (same clone as 60299-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60299-1-Ig (ICAM-1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 60299-1-Ig (same clone as 60299-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60299-1-Ig (ICAM-1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Daudi using 60299-1-Ig (same clone as 60299-1-PBS)

  1X10^6 Daudi cells were surface stained with 0.4 ug Anti-Human ICAM-1 (60299-1-Ig, Clone:2F9A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed. This data was developed using the same antibody clone with 60299-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60299-1-PBS targets ICAM-1 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ICAM-1 fusion protein Ag8309
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC015969
• Gene Symbol: ICAM1
• Gene ID (NCBI): 3383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes¹. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-1². In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions^{3, 4}. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material⁵.

1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).