Rat ICAM-1/CD54 Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00678-2

  Sandwich ELISA standard curve of MP00678-2, Rat ICAM-1/CD54 Recombinant Matched Antibody Pair - PBS only. 83696-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1025. 83696-2-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00678-2

  Rat heart conditioned media (1 heart, 1-2 mm pieces) was cultured for 4 days in 30 mL of RPMI supplemented with 10% fetal bovine serum and stimulated with 2.5 ng/mL LPS. The mean ICAM-1/CD54 concentration was determined to be 2,606.5 pg/mL in stimulated heart supernatant.
  Spleens from rats were removed, rinsed in PBS, and kept on ice. The tissue was homogenized using a tissue homogenizer and seeded into media containing DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL of streptomycin sulfate, and 50 ng/mL gentamicin solution. Cells were culture stimulated with 50 ng/mL of recombinant rat IL-2 and 50 ng/ml PHA for 3 days. The mean ICAM-1/CD54 concentration was determined to be 423.9 pg/mL in stimulated spleen supernatant.

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  Sample test of MP00678-2

  Serum of sixteen rats was measured. The ICAM-1/CD54 concentration of detected samples was determined to be 15.3 ng/mL with a range of 12.9 - 20.5 ng/mL.

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  Cytometric bead array standard curve of MP00678-2

  Cytometric bead array standard curve of MP00678-2, RAT ICAM-1/CD54 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83696-4-PBS. Detection antibody: 83696-2-PBS. Standard: Eg1025. Range: 0.156-20 ng/mL.

ICAM-1/CD54 {ptg:Brand} Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83696-4-PBS

Clone No.240725E10

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD54, ICAM-1, ICAM-12, Icam1, 240725E10

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Product Information

83696-4-PBS targets ICAM-1/CD54 as part of a matched antibody pair:

MP00678-2: 83696-4-PBS capture and 83696-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: NM_012967.1
• Gene Symbol: Icam1
• Gene ID (NCBI): 25464
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q00238
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.