FcZero-rAb® PE Anti-Rat ICAM-1/CD54 Rabbit Recombinant Antibody

• Home
• Products
• Primary Antibodies
• Flow Cytometry Antibodies
• FcZero-rAb® PE Anti-Rat ICAM-1/CD54 Rabbit Recombinant Antibody (PE-FcA98060)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

ICAM-1/CD54 FcZero-rAb^® Recombinant Antibody for FC

Cat No. PE-FcA98060 Copied!

Clone No. 240725C2

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC

Synonyms

CD54, ICAM-1, Icam1

• 

  FC experiment of rat splenocytes using PE-FcA98060

  1x10^6 rat splenocytes were surface stained with 0.06 ug PE Anti-Rat ICAM-1/CD54 Rabbit RecAb (PE-FcA98060, Clone: 240725C2) (red) or PE Rabbit IgG Isotype Control RecAb (PE-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for ICAM-1/CD54 in ?

Chat with Able

---

Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.06 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98060 targets ICAM-1/CD54 in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1025

  Product name: Recombinant rat ICAM-1/CD54 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 28-493 aa of NM_012967.1

  Sequence: QVSIHPTEAFLPRGGSVQVNCSSSCEDENLGLGLETNWMKDELSSGHNWKLFKLSDIGEDSRPLCFENCGTTQSSASATITVYSFPERVELDPLPAWQQVGKNLILRCLVEGGAPRTQLSVVLLRGNETLSRQAVDGDPKEITFTVLASRGDHGANFSCFTELDLRPQGLSLFKNVSEVRQLRTFDLPTRVLKLDTPDLLEVGTQQKFLCSLEGLFPASEAQIYLEMGGQMLTLESTNSRDFVSATASVEVTEKLDRTLQLRCVLELADQTLEMEKTLRIYNFSAPILTLSQPEVSEGDQVTVKCEAHGGAQVVLLNSTSPRPPTSQGTSPRPPTSQIQFTLNASPEDHKRRFFCSAALEVDGKSLFKNQTLELHVLYGPHLDKKDCLGNWTWQEGSQQTLTCQPQGNPAPNLTCSRKADGVPLPIGMVKSVKREMNGTYKCRAFSSRGSITRDVHLTVLYHDQNT

• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: NM_012967.1
• Gene Symbol: Icam1
• Gene ID (NCBI): 25464
• RRID: AB_3749723
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Excitation Laser: Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q00238
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).