CoraLite®594 Anti-Human ICAM-2/CD102 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL594-98367

  1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD45, and 5 ul CoraLite®594 Anti-Human ICAM-2/CD102 Rabbit RecAb (CL594-98367, Clone: 242237F8) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9).Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL594-98367

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human ICAM-2/CD102 Rabbit RecAb (CL594-98367, Clone: 242237F8) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9)(blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

ICAM-2/CD102 Uni-rAb^® Recombinant Antibody for FC

Cat No. CL594-98367

Clone No.242237F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD102, ICAM2, 242237F8, ICAM 2, ICAM-2

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98367 targets ICAM-2/CD102 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2002

  Product name: Recombinant Human ICAM2/CD102 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 25-223 aa of NM_001099789.2

  Sequence: KVFEVHVRPKKLAVEPKGSLEVNCSTTCNQPEVGGLETSLDKILLDEQAQWKHYLVSNISHDTVLQCHFTCSGKQESMNSNVSVYQPPRQVILTLQPTLVAVGKSFTIECRVPTVEPLDSLTLFLFRGNETLHYETFGKAAPAPQEATATFNSTADREDGHRNFSCLAVLDLMSRGGNIFHKHSAPKMLEIYEPVSDSQ

• Full Name: intercellular adhesion molecule 2
• Calculated Molecular Weight: 31kDa
• GenBank Accession Number: NM_001099789.2
• Gene Symbol: ICAM2/CD102
• Gene ID (NCBI): 3384
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13598
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.