Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of human PBMCs using 98097-1-RR (same clone as 98097-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98097-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98097-1-RR (same clone as 98097-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98097-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98097-1-RR (same clone as 98097-1-PBS)

  1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody (98097-1-RR, Clone:241418E8) and PE-conjugated Goat Anti-Rabbit IgG, and 0.25 ug Isotype Control. Cells were not fixed. This data was developed using the same antibody clone with 98097-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98097-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98097-1-RR against Human ICAM3/CD50 were performed. The affinity constant is 0.366 nM.

ICAM3/CD50 Uni-rAb^® Recombinant Antibody for FC, Indirect ELISA

Cat No. 98097-1-PBS

Clone No.241418E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Indirect ELISA

Synonyms

CDW50, ICAM3, 241418E8, CD50, ICAM 3

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Product Information

98097-1-PBS targets ICAM3/CD50 in FC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: intercellular adhesion molecule 3
• Calculated Molecular Weight: 547 aa, 60 kDa
• GenBank Accession Number: BC058903
• Gene Symbol: ICAM3/CD50
• Gene ID (NCBI): 3385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P32942
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The intercellular adhesion molecule (ICAM) family is a subfamily of immunoglobulin superfamily with five known ICAM family members (ICAM1 to ICAM5) which play a role in inflammation, immune responses, and intracellular signaling (PMID: 23287428; 29477378). ICAM3, also known as CD50, is a type I transmembrane glycoprotein that contains five C2-type immunoglobulin-like domains in its extracellular region, a single transmembrane segment, and a C-terminus cytoplasmic tail. ICAM3 is selectively expressed on leukocytes (PMID: 1730916; 8132828). It is the primary ligand for LFA-1 and is involved in various immune processes, including T-cell activation, and macrophage recognition of apoptotic cells (PMID: 11812993; 23775590).