CoraLite® Plus 647 Anti-Human ICAM3/CD50 Rabbit Recombinant Antibody

• 

  FC experiment of human PBMCs using CL647-98097

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human ICAM3/CD50 Rabbit RecAb (CL647-98097, Clone: 241418E8) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL647-98097

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human ICAM3/CD50 Rabbit RecAb (CL647-98097, Clone: 241418E8)(red), or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue).Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

ICAM3/CD50 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98097

Clone No.241418E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CDW50, ICAM3, 241418E8, CD50, ICAM 3

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98097 targets ICAM3/CD50 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: intercellular adhesion molecule 3
• Calculated Molecular Weight: 547 aa, 60 kDa
• GenBank Accession Number: BC058903
• Gene Symbol: ICAM3/CD50
• Gene ID (NCBI): 3385
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P32942
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The intercellular adhesion molecule (ICAM) family is a subfamily of immunoglobulin superfamily with five known ICAM family members (ICAM1 to ICAM5) which play a role in inflammation, immune responses, and intracellular signaling (PMID: 23287428; 29477378). ICAM3, also known as CD50, is a type I transmembrane glycoprotein that contains five C2-type immunoglobulin-like domains in its extracellular region, a single transmembrane segment, and a C-terminus cytoplasmic tail. ICAM3 is selectively expressed on leukocytes (PMID: 1730916; 8132828). It is the primary ligand for LFA-1 and is involved in various immune processes, including T-cell activation, and macrophage recognition of apoptotic cells (PMID: 11812993; 23775590).