ICT1 Monoclonal antibody, PBS Only

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  WB analysis using 68898-3-Ig (same clone as 68898-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68898-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 68898-3-Ig (same clone as 68898-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68898-3-PBS in a different storage buffer formulation.

• 

  WB analysis of SW480 using 68898-3-Ig (same clone as 68898-3-PBS)

  SW480 cells were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68898-3-PBS in a different storage buffer formulation.

• 

  IF Staining of Caco-2 using 68898-3-Ig (same clone as 68898-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ICT1 antibody (68898-3-Ig, Clone: 1F11E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68898-3-PBS in a different storage buffer formulation.

ICT1 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68898-3-PBS

Clone No.1F11E6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

1F11E6, 39S ribosomal protein L58, mitochondrial, DS1, DS-1, EC:3.1.1.29

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Product Information

68898-3-PBS targets ICT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33109

  Product name: Recombinant human ICT1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-206 aa of BC015335

  Sequence: MAATRCLRWGLSRAGVWLLPPPARCPRRALHKQKDGTEFKSIYSLDKLYPESQGSDTAWRVPNGAKQADSDIPLDRLTISYCRSSGPGGQNVNKVNSKAEVRFHLATAEWIAEPVRQKIAITHKNKINRLGELILTSESSRYQFRNLADCLQKIRDMITEASQTPKEPTKEDVKLHRIRIENMNRERLRQKRIHSAVKTSRRVDMD

• Full Name: immature colon carcinoma transcript 1
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 21-24 kDa
• GenBank Accession Number: BC015335
• Gene Symbol: ICT1
• Gene ID (NCBI): 3396
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q14197
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than differentiated cells.