Tested Applications
| Positive WB detected in | HCT 116 cells, Calu-3 cells, SW480 cells, LNCaP cells, HeLa cells, HEK-293 cells, SK-BR-3 cells |
| Positive IF/ICC detected in | Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68898-3-Ig targets ICT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33109 Product name: Recombinant human ICT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-206 aa of BC015335 Sequence: MAATRCLRWGLSRAGVWLLPPPARCPRRALHKQKDGTEFKSIYSLDKLYPESQGSDTAWRVPNGAKQADSDIPLDRLTISYCRSSGPGGQNVNKVNSKAEVRFHLATAEWIAEPVRQKIAITHKNKINRLGELILTSESSRYQFRNLADCLQKIRDMITEASQTPKEPTKEDVKLHRIRIENMNRERLRQKRIHSAVKTSRRVDMD Predict reactive species |
| Full Name | immature colon carcinoma transcript 1 |
| Calculated Molecular Weight | 24 kDa |
| Observed Molecular Weight | 21-24 kDa |
| GenBank Accession Number | BC015335 |
| Gene Symbol | ICT1 |
| Gene ID (NCBI) | 3396 |
| RRID | AB_3670448 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q14197 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than differentiated cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ICT1 antibody 68898-3-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
