Tested Applications
| Positive WB detected in | HeLa cells, A375 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, A431 cells, HEK-293 cells, MCF-7 cells, NCCIT cells, HT-1080 cells, LNCaP cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
67106-1-Ig targets IDE in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17161 Product name: Recombinant human IDE protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 676-1019 aa of BC096336 Sequence: EQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL Predict reactive species |
| Full Name | Abeta degrading protease |
| Calculated Molecular Weight | 1019 aa, 118 kDa |
| Observed Molecular Weight | 100-120 kDa |
| GenBank Accession Number | BC096336 |
| Gene Symbol | IDE |
| Gene ID (NCBI) | 3416 |
| RRID | AB_2882410 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P14735 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IDE(INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IDE antibody 67106-1-Ig | Download protocol |
| WB protocol for IDE antibody 67106-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging Cell Microglia-derived Galectin-9 drives amyloid-β pathology in Alzheimer's disease | ||
Mol Nutr Food Res Long-Term High-Fat Diet Decreases Renal Insulin-Degrading Enzyme Expression and Function by Inhibiting the PPARγ Pathway | ||
Mol Med Inhibition of insulin degrading enzyme suppresses osteoclast hyperactivity via enhancing Nrf2-dependent antioxidant response in glucocorticoid-induced osteonecrosis of the femoral head | ||
Phytomedicine Osmundacetone ameliorates Alzheimer's-like pathologies by inhibiting β-amyloid fibrillation, oxidative damage and neuroinflammation in APP/PS1 transgenic mice | ||
Phytomedicine Jiawei Xionggui Decoction promotes meningeal lymphatic vessels clearance of β-amyloid by inhibiting arachidonic acid pathway |
