Tested Applications
| Positive WB detected in | HepG2 cells, human skeletal muscle tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15909-1-AP targets IDH3A in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8662 Product name: Recombinant human IDH3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC021967 Sequence: MAGPAWISKVSRLLGAFHNPKQVTRGFTGGVQTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD Predict reactive species |
| Full Name | isocitrate dehydrogenase 3 (NAD+) alpha |
| Calculated Molecular Weight | 366 aa, 40 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC021967 |
| Gene Symbol | IDH3A |
| Gene ID (NCBI) | 3419 |
| RRID | AB_2123282 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50213 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IDH3A antibody 15909-1-AP | Download protocol |
| IF protocol for IDH3A antibody 15909-1-AP | Download protocol |
| IHC protocol for IDH3A antibody 15909-1-AP | Download protocol |
| IP protocol for IDH3A antibody 15909-1-AP | Download protocol |
| WB protocol for IDH3A antibody 15909-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-IDH3 antibody (Cat# 15909-1-AP) has 35 citations across journals including Nature Neuroscience, Nature Communications, Molecular Cell, Cell Reports, Cell Death & Disease, EMBO Journal, Diabetes, and others. Research fields encompass mitochondrial metabolism, cancer (prostate, breast, melanoma, HCC), neurodegeneration, diabetes, obesity, cardiovascular disease, immunology, and proteomics. Primary applications include Western blot, immunohistochemistry, and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Nat Commun Dysregulation of mitochondrial α-ketoglutarate dehydrogenase leads to elevated lipid peroxidation in CHCHD2-linked Parkinson's disease models | ||
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. | ||
Mol Cell Accumulation of succinate suppresses de novo purine synthesis through succinylation-mediated control of the mitochondrial folate cycle. | ||
Cell Rep Med Acutely denervated muscle EVs reshape neuronal mitochondrial metabolism via retrograde signaling to rescue peripheral nerve injury. | ||
Cell Death Dis c-Fos-driven metabolic switch of α-ketoglutarate orchestrates progression in prostate cancer.
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Reviews
What customers say
The single review for 15909-1-AP is a 5-star rating from a Western Blot application on human and mouse brain tissues. The reviewer used a 1:1000 dilution with 15 µg protein per lane on 4–12% Bis-Tris gels, overnight transfer at 30V, and SEA Block for blocking. The antibody produced clear, specific bands across multiple samples, including human and mouse cortex homogenates and non-synaptosomal mitochondria, confirming reliable performance for detecting the target in both species.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Svitlana (Verified Customer) (03-19-2019) | SDS-PAGE: 15 ug protein lysate/well, 4-12% Bis-Tris.Transfer: Immobilon-FL transfer membranes O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1h at room temperature.Secondary Ab: IRDye 800CW Goat anti-Rabbit 1 h at room temperature.Lines on WB1. BioRad Precision Plus Protein standard2. Homogenate of human cortex tissue3. Non-synaptosomal mitochondria from human cortex4. Homogenate of mice brain tissue5. Non-synaptosomal mitochondria from mice brains
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