• Featured Product
  • KD/KO Validated

IDH3A Polyclonal antibody

IDH3A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15909-1-AP

Synonyms

IDH3A



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, HepG2 cells, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15909-1-AP targets IDH3A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IDH3A fusion protein Ag8662
Full Name isocitrate dehydrogenase 3 (NAD+) alpha
Calculated molecular weight 366 aa, 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC021967
Gene symbol IDH3A
Gene ID (NCBI) 3419
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IDH3A antibody 15909-1-APDownload protocol
IHC protocol for IDH3A antibody 15909-1-APDownload protocol
IF protocol for IDH3A antibody 15909-1-APDownload protocol
IP protocol for IDH3A antibody 15909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Dis Model Mech

Mouse Idh3a mutations cause retinal degeneration and reduced mitochondrial function.

Authors - Amy S Findlay
  • KO Validated
ratIF

Sci Rep

Complement component 1q subcomponent binding protein in the brain of the rat.

Authors - János Barna
mouseIF

Cell Rep

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.

Authors - Noemi Holderith
humanWB

Circ J

Does the Rewarmed Heart Restore the Myocardial Proteome to That of the Pre-Cooled State? - A Proteomic Analysis of Surgical Samples.

Authors - Teiji Oda
mouseWB,IHC

Biochem Biophys Res Commun

Ifi27 is indispensable for mitochondrial function and browning in adipocytes.

Authors - Weiwei Jin
mouseWB, IF

J Proteomics

Synaptic mitochondria: A brain mitochondria cluster with a specific proteome.

Authors - Katalin Völgyi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Svitlana (Verified Customer) (03-19-2019)

SDS-PAGE: 15 ug protein lysate/well, 4-12% Bis-Tris. Transfer: Immobilon-FL transfer membranes O/N at 30V, 4C Blocking: SEA Block Blocking Buffer 1h at room temperature. Secondary Ab: IRDye 800CW Goat anti-Rabbit 1 h at room temperature. Lines on WB 1. BioRad Precision Plus Protein standard 2. Homogenate of human cortex tissue 3. Non-synaptosomal mitochondria from human cortex 4. Homogenate of mice brain tissue 5. Non-synaptosomal mitochondria from mice brains

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissues, human and mouse
IDH3A Antibody Western Blot, validation (1:1000 dilution) in Brain tissues, human and mouse (Cat no:15909-1-AP)