CoraLite® Plus 488-conjugated IDS Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated IDS Recombinant monoclonal antibody (CL488-84623-7)

IDS Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-84623-7

Clone No.242054G12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

242054G12, Alpha-L-iduronate sulfate sulfatase, EC:3.1.6.13, Iduronate 2-sulfatase, Iduronate 2-sulfatase 14 kDa chain

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  IF Staining of HeLa using CL488-84623-7

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 IDS antibody (CL488-84623-7, Clone: 242054G12 ) at dilution of 1:1000.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84623-7 targets IDS in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag31132

  Product name: Recombinant human IDS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 160-312 aa of BC006170

  Sequence: PSSEKYENTKTCRGPDGELHANLLCPVDVLDVPEGTLPDKQSTEQAIQLLEKMKTSASPFFLAVGYHKPHIPFRYPKEFQKLYPLENITLAPDPEVPDGLPPVAYNPWMDIRQREDVQALNISVPYGPIPVDFQEDQSSTGFRLKTSSTRKYK

• Full Name: iduronate 2-sulfatase
• Calculated Molecular Weight: 312aa,35 kDa; 550aa,62 kDa
• GenBank Accession Number: BC006170
• Gene Symbol: IDS
• Gene ID (NCBI): 3423
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P22304
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).