IDUA Polyclonal antibody, PBS Only

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• IDUA Polyclonal antibody, PBS Only (55158-1-PBS)

• 

  WB analysis using 55158-1-AP (same clone as 55158-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55158-1-AP (IDUA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55158-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 55158-1-AP (same clone as 55158-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55158-1-AP (IDUA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55158-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 55158-1-AP (same clone as 55158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55158-1-AP (IDUA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55158-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 55158-1-AP (same clone as 55158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55158-1-AP (IDUA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55158-1-PBS in a different storage buffer formulation.

IDUA Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 55158-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

55158-1-PBS targets IDUA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: iduronidase, alpha-L-
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: NM_000203
• Gene Symbol: IDUA
• Gene ID (NCBI): 3425
• RRID: AB_11182946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35475
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IDUA belongs to the glycosyl hydrolase 39 family. It hydrolysis of unsulfated alpha-L-iduronosidic linkages in dermatan sulfate. Defects in IDUA are the cause of mucopolysaccharidosis type 1H (MPS1H)( also known as Hurler syndrome), MPS1H/S(also known as Hurler-Scheie syndrome) and MPS1S(also known as Scheie syndrome), which are rare lysosomal storage diseases. This antibody is specific to IDUA.