IFI16 Polyclonal antibody

IFI16 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 29280-1-AP


Ifi 16, IFI16, IFNGIP1, PYHIN2

Tested Applications

Positive WB detected inDaudi cells, Jurkat cells, MOLT-4 cells, Ramos cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29280-1-AP targets IFI16 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFI16 fusion protein Ag30297
Full Name interferon, gamma-inducible protein 16
Calculated Molecular Weight 729 aa, 82 kDa
Observed Molecular Weight85-95 kDa
GenBank Accession NumberBC017059
Gene Symbol IFI16
Gene ID (NCBI) 3428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response. IFI16 has different isoforms and 67790-1-Ig antibody detects proteins around 85-95 kDa in SDS-PAGE similar to papers. (PMID: 9718316, 14990579, 11146555, 22046441)


Product Specific Protocols
WB protocol for IFI16 antibody 29280-1-APDownload protocol
IHC protocol for IFI16 antibody 29280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols