IFI16 Monoclonal antibody

IFI16 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1C10D7

Cat no : 67790-1-Ig

Synonyms

Ifi 16, IFI16, IFNGIP1, PYHIN2



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, MOLT-4 cells, Ramos cells, Daudi cells
Positive IHC detected inhuman tonsillitis tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

67790-1-Ig targets IFI16 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFI16 fusion protein Ag30314
Full Name interferon, gamma-inducible protein 16
Calculated molecular weight 729 aa, 82 kDa
Observed molecular weight85-95 kDa
GenBank accession numberBC017059
Gene symbol IFI16
Gene ID (NCBI) 3428
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response. IFI16 has different isoforms and 67790-1-Ig antibody detects proteins around 85-95 kDa in SDS-PAGE similar to papers. (PMID: 9718316, 14990579, 11146555, 22046441)

Protocols

Product Specific Protocols
WB protocol for IFI16 antibody 67790-1-IgDownload protocol
IHC protocol for IFI16 antibody 67790-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols