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IFITM1-Specific Monoclonal antibody
IFITM1-Specific Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 60074-1-Ig
Validation Data Gallery
|Positive WB detected in
|TF-1 cells, K-562 cells, human testis tissue, HepG2 cells, HL-60 cells
|Positive IP detected in
|Positive IHC detected in
|human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:20000-1:100000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:200-1:1000
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
60074-1-Ig targets IFITM1-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Mouse / IgG2a
|IFITM1-Specific fusion protein Ag2320
|interferon induced transmembrane protein 1 (9-27)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.
|Product Specific Protocols
|WB protocol for IFITM1-Specific antibody 60074-1-Ig
|IHC protocol for IFITM1-Specific antibody 60074-1-Ig
|IF protocol for IFITM1-Specific antibody 60074-1-Ig
|IP protocol for IFITM1-Specific antibody 60074-1-Ig
|FC protocol for IFITM1-Specific antibody 60074-1-Ig
|Click here to view our Standard Protocols
IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.
The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.
Cell Stem Cell
Sci Transl Med
Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition.
Cell Host Microbe
Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Claudia (Verified Customer) (06-20-2022)
The antibody worked very well for the analysis of IFITM1 expression in permanent cell lines.