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  • KD/KO Validated

IFITM1-Specific Monoclonal antibody

IFITM1-Specific Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

5B5E2

Cat no : 60074-1-Ig

Synonyms

9 27, CD225, IFI17, IFITM1, IFITM1-Specific, Interferon induced protein 17, Leu 13 antigen, LEU13



Tested Applications

Positive WB detected inTF-1 cells, K-562 cells, human testis tissue, HepG2 cells, HL-60 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inK-562 cells
Positive FC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60074-1-Ig targets IFITM1-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IFITM1-Specific fusion protein Ag2320
Full Name interferon induced transmembrane protein 1 (9-27)
Calculated molecular weight 14 kDa
Observed molecular weight 14-17 kDa
GenBank accession numberBC000897
Gene symbol IFITM1
Gene ID (NCBI) 8519
RRIDAB_2233405
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.

Protocols

Product Specific Protocols
WB protocol for IFITM1-Specific antibody 60074-1-IgDownload protocol
IHC protocol for IFITM1-Specific antibody 60074-1-IgDownload protocol
IF protocol for IFITM1-Specific antibody 60074-1-IgDownload protocol
IP protocol for IFITM1-Specific antibody 60074-1-IgDownload protocol
FC protocol for IFITM1-Specific antibody 60074-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,FC

Science

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Authors - Julian Buchrieser
humanWB

Cell

The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.

Authors - Abraham L Brass
humanWB

Cell Stem Cell

PMID: 30416070

Authors - Carolina Petrillo
humanWB

Sci Transl Med

Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition.

Authors - Mohammed Selman
humanWB

Cell Host Microbe

Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.

Authors - Toshana L Foster
  • KD Validated
humanIF

Gastroenterology

PMID: 23123437

Authors - Daryl T-Y Lau

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Claudia (Verified Customer) (06-20-2022)

The antibody worked very well for the analysis of IFITM1 expression in permanent cell lines.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:400 (immunofluorescence), 1:20.000 (Western blot)
  • Cell Tissue Type: Permanent cell lines