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  • KD/KO Validated

IFITM2 Polyclonal antibody

IFITM2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12769-1-AP

Synonyms

1 8D, IFITM2



Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12769-1-AP targets IFITM2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFITM2 fusion protein Ag3451
Full Name interferon induced transmembrane protein 2 (1-8D)
Calculated molecular weight 132 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC009696
Gene symbol IFITM2
Gene ID (NCBI) 10581
RRIDAB_2122089
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. Catalog#12769-1-AP is a rabbit polyclonal antibody produced with full-length of human IFITM2.

Protocols

Product Specific Protocols
WB protocol for IFITM2 antibody 12769-1-APDownload protocol
IHC protocol for IFITM2 antibody 12769-1-APDownload protocol
IF protocol for IFITM2 antibody 12769-1-APDownload protocol
IP protocol for IFITM2 antibody 12769-1-APDownload protocol
FC protocol for IFITM2 antibody 12769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.

Authors - Abraham L Brass
humanWB

Cell Stem Cell

PMID: 30416070

Authors - Carolina Petrillo
humanWB

Cell Host Microbe

Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.

Authors - Toshana L Foster
  • KD Validated
humanWB, FC

Cell Host Microbe

IFITM Proteins Incorporated into HIV-1 Virions Impair Viral Fusion and Spread.

Authors - Alex A Compton
  • KD Validated
humanWB

Nat Commun

IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses

Authors - M. Clement
mouseWB

Nat Commun

BAG3 promotes pancreatic ductal adenocarcinoma growth by activating stromal macrophages.

Authors - Alessandra Rosati
  • KD Validated