|Positive WB detected in||MCF-7 cells, human lung tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human breast cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12769-1-AP targets IFITM2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFITM2 fusion protein Ag3451|
|Full Name||interferon induced transmembrane protein 2 (1-8D)|
|Calculated molecular weight||132aa,15 kDa|
|Observed molecular weight||15 kDa|
|GenBank accession number||BC009696|
|Gene ID (NCBI)||10581|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. Catalog#12769-1-AP is a rabbit polyclonal antibody produced with full-length of human IFITM2.
|Product Specific Protocols|
|WB protocol for IFITM2 antibody 12769-1-AP||Download protocol|
|IHC protocol for IFITM2 antibody 12769-1-AP||Download protocol|
|IF protocol for IFITM2 antibody 12769-1-AP||Download protocol|
|IP protocol for IFITM2 antibody 12769-1-AP||Download protocol|
|Click here to view our Standard Protocols|
IFITMs mediate viral evasion in acute and chronic hepatitis C virus infection.
J Infect Dis
Interferon-Induced Transmembrane Protein-Mediated Inhibition of Host Cell Entry of Ebolaviruses.
Role of rhesus macaque IFITM3(2) in simian immunodeficiency virus infection of macaques.
J Gen Virol
Respiratory Syncytial Virus Infection is Inhibited by Interferon-Induced Transmembrane Proteins.
Antiviral Role of IFITM Proteins in African Swine Fever Virus Infection.
IFITM Proteins Inhibit Entry Driven by the MERS-Coronavirus Spike Protein: Evidence for Cholesterol-Independent Mechanisms.