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- KD/KO Validated
IFITM2 Polyclonal antibody
IFITM2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 12769-1-AP
Validation Data Gallery
|Positive WB detected in
|HepG2 cells, MCF-7 cells
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|IF : 1:20-1:200
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
12769-1-AP targets IFITM2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, pig
|Host / Isotype
|Rabbit / IgG
|IFITM2 fusion protein Ag3451
|interferon induced transmembrane protein 2 (1-8D)
|Calculated molecular weight
|132 aa, 15 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. Catalog#12769-1-AP is a rabbit polyclonal antibody produced with full-length of human IFITM2.
|Product Specific Protocols
|WB protocol for IFITM2 antibody 12769-1-AP
|IHC protocol for IFITM2 antibody 12769-1-AP
|IF protocol for IFITM2 antibody 12769-1-AP
|IP protocol for IFITM2 antibody 12769-1-AP
|FC protocol for IFITM2 antibody 12769-1-AP
|Click here to view our Standard Protocols
The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.
Cell Stem Cell
Cell Host Microbe
Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.
Cell Host Microbe
IFITM Proteins Incorporated into HIV-1 Virions Impair Viral Fusion and Spread.
IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses
BAG3 promotes pancreatic ductal adenocarcinoma growth by activating stromal macrophages.