CoraLite® Plus 488-conjugated IFITM2 Polyclonal antibody

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• CoraLite® Plus 488-conjugated IFITM2 Polyclonal antibody (CL488-12769)

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  IF Staining of HeLa using CL488-12769

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 IFITM2 antibody (CL488-12769) at dilution of 1:200, CL594-phalloidin (red).

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  FC experiment of MCF-7 using CL488-12769

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated IFITM2 Polyclonal antibody (CL488-12769)(red), or 0.8 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

• Featured Product
• KD/KO Validated

IFITM2 Polyclonal Antibody for IF/ICC

Cat No. CL488-12769

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

1 8D, 18D, DSPA2c, IFITM 2, Interferon-induced transmembrane protein 2

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.8 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12769 targets IFITM2 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3451

  Product name: Recombinant human IFITM2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-132 aa of BC009696

  Sequence: MNHIVQTFSPVNSGQPPNYEMLKEEQEVAMLGAPHNPAPPTSTVIHIRSETSVPDHVVWSLFNTLFMNTCCLGFIAFAYSVKSRDRKMVGDVTGAQAYASTAKCLNIWALILGIFMTILLVIIPVLVVQAQR

• Full Name: interferon induced transmembrane protein 2 (1-8D)
• Calculated Molecular Weight: 132 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC009696
• Gene Symbol: IFITM2
• Gene ID (NCBI): 10581
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01629
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. Catalog#12769-1-AP is a rabbit polyclonal antibody produced with full-length of human IFITM2.