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- KD/KO Validated
IFITM3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11714-1-AP
1 8U, Fragilis, IFITM3, IP15
Validation Data Gallery
|Positive WB detected in||HeLa cells, mouse colon tissue, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human lung cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11714-1-AP targets IFITM3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||goat, human, mouse, rat, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFITM3 fusion protein Ag2285|
|Full Name||interferon induced transmembrane protein 3 (1-8U)|
|Calculated molecular weight||133aa,15 kDa|
|Observed molecular weight||14 kDa|
|GenBank accession number||BC006794|
|Gene ID (NCBI)||10410|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. Catalog#11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3.
|Product Specific Protocols|
|WB protocol for IFITM3 antibody 11714-1-AP||Download protocol|
|IHC protocol for IFITM3 antibody 11714-1-AP||Download protocol|
|IF protocol for IFITM3 antibody 11714-1-AP||Download protocol|
|IP protocol for IFITM3 antibody 11714-1-AP||Download protocol|
|FC protocol for IFITM3 antibody 11714-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Nat Chem Biol
IFITM3 directly engages and shuttles incoming virus particles to lysosomes.
Int J Biol Macromol
Construction, expression and antiviral activity analysis of recombinant adenovirus expressing human IFITM3 in vitro.
Vpr Enhances HIV-1 Env Processing and Virion Infectivity in Macrophages by Modulating TET2-Dependent IFITM3 Expression.
Cell Lineage Tracing Identifies Hormone-Regulated and Wnt-Responsive Vaginal Epithelial Stem Cells.
Cell Chem Biol
Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.
Res Vet Sci
Infection of goats with goatpox virus triggers host antiviral defense through activation of innate immune signaling.