IFNA8 Recombinant monoclonal antibody

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• IFNA8 Recombinant monoclonal antibody (87827-1-RR)

• 

  WB analysis using 87827-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87827-1-RR (IFNA8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity of 87827-1-RR

  Biolayer interferometry (BLI) kinetic assays of 87827-1-RR against Human IFNA8 were performed. The affinity constant is 0.54 nM.

IFNA8 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87827-1-RR

Clone No.252963A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IFN-alpha-8, Interferon Alpha 8, Interferon alpha-8, Interferon alpha-B, Interferon alpha-B2

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Tested Applications

• Positive WB detected in: Transfected with IFNA8 HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87827-1-RR targets IFNA8 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4954

  Product name: Recombinant Human IFNA8 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 24-189 aa of BC104830

  Sequence: CDLPQTHSLGNRRALILLAQMRRISPFSCLKDRHDFEFPQEEFDDKQFQKAQAISVLHEMIQQTFNLFSTKDSSAALDETLLDEFYIELDQQLNDLESCVMQEVGVIESPLMYEDSILAVRKYFQRITLYLTEKKYSSCAWEVVRAEIMRSFSLSINLQKRLKSKE

• Full Name: interferon, alpha 8
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC104830
• Gene Symbol: IFNA8
• Gene ID (NCBI): 3445
• RRID: AB_3745773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P32881
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFNA8 (Interferon alpha-8) is a member of the type I interferon family, primarily produced by macrophages and virus-infected cells, and possesses broad-spectrum antiviral activity. This protein exerts its effects by binding to the type I interferon receptor, activating the JAK-STAT signaling pathway, and inducing the expression of various interferon-stimulated genes (ISGs). Studies have shown that the antiviral activity of IFNA8 is significantly higher than that of other subtypes such as IFNA1, playing a crucial role in defending against infections like vesicular stomatitis virus. Furthermore, the transcription of IFNA8 is regulated by the transcription factor Oct-1, and single nucleotide polymorphisms in its promoter region are associated with the prognosis of glioma patients.