Anti-Human IFNAR2 Rabbit Recombinant Antibody

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• Anti-Human IFNAR2 Rabbit Recombinant Antibody (98474-5-RR)

• 

  FC experiment of human blood using 98474-5-RR

  1x10^6 human blood were surface stained with 0.25 ug Anti-Human IFNAR2 Rabbit RecAb (98474-5-RR, Clone: 250313D4) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human blood using 98474-5-RR

  1x10^6 human blood cells were surface stained with 0.25 ug Anti-Human IFNAR2 Rabbit RecAb (98474-5-RR, Clone: 250313D4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity of 98474-5-RR

  Biolayer interferometry (BLl) kinetic assays of 98474-5-RR against Human IFNAR2 were performed. The affinity constant is below 1 pM.

IFNAR2 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98474-5-RR

Clone No.250313D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

IFN alpha REC, IFN alpha/beta receptor 2, IFN R, IFN R 2, IFNABR

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Tested Applications

• Positive FC detected in: human blood cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98474-5-RR targets IFNAR2 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2928

  Product name: Recombinant Human IFNAR2 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 27-243 aa of NM_207585.2

  Sequence: ISYDSPDYTDESCTFKISLRNFRSILSWELKNHSIVPTHYTLLYTIMSKPEDLKVVKNCANTTRSFCDLTDEWRSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEELQFDLSLVIEEQSEGIVKKHKPEIKGNMSGNFTYIIDKLIPNTNYCVSVYLEHSDEQAVIKSPLKCTLLPPGQESESAESAK

• Full Name: interferon (alpha, beta and omega) receptor 2
• Calculated Molecular Weight: 58 kDa
• GenBank Accession Number: NM_207585.2
• Gene Symbol: IFNAR2
• Gene ID (NCBI): 3455
• RRID: AB_3746104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48551-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Interferon alpha/beta receptor 2 (IFNAR2) is a type I membrane protein that associates with IFNAR1 to form the receptor for type I interferons. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.