IFT122 Polyclonal antibody

IFT122 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 19304-1-AP

Synonyms

IFT122, SPG, WDR10, WDR10p, WDR140



Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 19304-1-AP is IFT122 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT122 fusion protein Ag6860
Full Name intraflagellar transport 122 homolog (Chlamydomonas)
Calculated molecular weight 142 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberBC004238
Gene symbol IFT122
Gene ID (NCBI) 55764
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IFT122 antibody 19304-1-APDownload protocol
IHC protocol for IFT122 antibody 19304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis.

Authors - Mariko Takahara
mouseWB

Cell

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

Authors - Siew Cheng Phua