IFT122 Polyclonal antibody

IFT122 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 19304-1-AP

Synonyms

IFT122, SPG, WDR10, WDR10p, WDR140



Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 19304-1-AP is IFT122 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT122 fusion protein Ag6860
Full Name intraflagellar transport 122 homolog (Chlamydomonas)
Calculated molecular weight 142 kDa
Observed molecular weight140 kDa
GenBank accession numberBC004238
Gene symbol IFT122
Gene ID (NCBI) 55764
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IFT122 antibody 19304-1-APDownload protocol
IHC protocol for IFT122 antibody 19304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis.

Authors - Mariko Takahara
mouseWB

Cell

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

Authors - Siew Cheng Phua