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WB analysis of mouse testis using 19304-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 19304-1-AP (IFT122 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 19304-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 19304-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human stomach using 19304-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach using 19304-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 19304-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 19304-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19304-1-AP (IFT122 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MDCK using 19304-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using IFT122 antibody (19304-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).
IF Staining of hTERT-RPE1 using 19304-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using IFT122 antibody (19304-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).
IF Staining of hTERT-RPE1 using 19304-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using IFT122 antibody (19304-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

IFT122 Polyclonal antibody

Name: IFT122 Polyclonal antibody
Brand: Proteintech
SKU: 19304-1-AP

IFT122 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19304-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

WDR10p, WDR10, WD repeat-containing protein 140, WD repeat-containing protein 10, SPG

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse testis tissue
Positive IHC detected in	human testis tissue, human brain tissue, human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MDCK cells, hTERT-RPE1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 1 publications below

Product Information

19304-1-AP targets IFT122 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.

Tested Reactivity	human, mouse, canine
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6860 Product name: Recombinant human IFT122 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-359 aa of BC004238 Sequence: MRAVLTWRDKAEHCINDIAFKPDGTQLILAAGSRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGLWSPEQKSVSKHKSSSKIICCSWTNDGQYLALGMFNGIISIRNKNGEEKVKIERPGGSLSPIWSICWNPSREERNDILAVADWGQKVSFYQLSGKQIGKDRALNFDPCCISYFTKGEYILLGGSDKQVSLFTKDGVRLGTVGEQNSWVWTCQAKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELVKKIAIYRNRLAIQLPEKILIYELYSEDLSDMHYRVKEKIIKKFECNLLVVCANHIILCQEKRLQCLSFSGVKEREWQME Predict reactive species
Full Name	intraflagellar transport 122 homolog (Chlamydomonas)
Calculated Molecular Weight	142 kDa
Observed Molecular Weight	140 kDa
GenBank Accession Number	BC004238
Gene Symbol	IFT122
Gene ID (NCBI)	55764
RRID	AB_2714184
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9HBG6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for IFT122 antibody 19304-1-AP	Download protocol
IHC protocol for IFT122 antibody 19304-1-AP	Download protocol
WB protocol for IFT122 antibody 19304-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision. Authors - Siew Cheng Phua View Article
mouse	WB,IF	Elife A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia. Authors - Tooba Quidwai View Article
human	WB	Hum Mol Genet Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis. Authors - Mariko Takahara View Article