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IFT57 Polyclonal antibody
IFT57 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat, Canine and More (1)
WB, IP, IHC, IF, ELISA
Cat no : 11083-1-AP
Validation Data Gallery
|Positive WB detected in||mouse retina tissue, HEK-293 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human prostate cancer tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 13 publications below|
|IF||See 10 publications below|
|IP||See 2 publications below|
11083-1-AP targets IFT57 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
|Tested Reactivity||human, mouse, rat, Canine|
|Cited Reactivity||human, mouse, Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFT57 fusion protein Ag1566|
|Full Name||intraflagellar transport 57 homolog (Chlamydomonas)|
|Calculated molecular weight||49 kDa|
|Observed molecular weight||49 kDa|
|GenBank accession number||BC011899|
|Gene ID (NCBI)||55081|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
IFT57, also named as DERP8, ESRRBL1, HIPPI and MHS4R2, belongs to the IFT57 family. It is required for the formation of cilia. IFT57 plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. It is a component of IFT complex B which composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. In contrast to BBS proteins, IFT57 and IFT88 is found in almost every cilium.(PMID:22072986)
|Product Specific Protocols|
|WB protocol for IFT57 antibody 11083-1-AP||Download protocol|
|IHC protocol for IFT57 antibody 11083-1-AP||Download protocol|
|IF protocol for IFT57 antibody 11083-1-AP||Download protocol|
|IP protocol for IFT57 antibody 11083-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Nat Cell Biol
The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.
Nat Cell Biol
Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.
The Intraflagellar Transport Protein IFT27 Promotes BBSome Exit from Cilia through the GTPase ARL6/BBS3.
IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.
Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.
Proc Natl Acad Sci U S A
Primary cilia signaling mediates intraocular pressure sensation.