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IFT57 Polyclonal antibody

IFT57 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11083-1-AP

Synonyms

DERP8, ESRRBL1, HIP1 interacting protein, HIPPI, IFT57, MHS4R2



Tested Applications

Positive WB detected inmouse retina tissue, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11083-1-AP targets IFT57 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivity Chicken, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT57 fusion protein Ag1566
Full Name intraflagellar transport 57 homolog (Chlamydomonas)
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC011899
Gene symbol IFT57
Gene ID (NCBI) 55081
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFT57, also named as DERP8, ESRRBL1, HIPPI and MHS4R2, belongs to the IFT57 family. It is required for the formation of cilia. IFT57 plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. It is a component of IFT complex B which composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. In contrast to BBS proteins, IFT57 and IFT88 is found in almost every cilium.(PMID:22072986)

Protocols

Product Specific Protocols
WB protocol for IFT57 antibody 11083-1-APDownload protocol
IHC protocol for IFT57 antibody 11083-1-APDownload protocol
IF protocol for IFT57 antibody 11083-1-APDownload protocol
IP protocol for IFT57 antibody 11083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Hum Mol Genet

Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.

Authors - Marie Alice Dupont
humanIF

Sci Rep

DYNC2LI1 mutations broaden the clinical spectrum of dynein-2 defects.

Authors - Kristin Kessler
humanWB

PLoS One

Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.

Authors - Yanan Xu
WB,IHC

Mol Biol Cell

Cooperation of the IFT-A complex with the IFT-B complex is required for ciliary retrograde protein trafficking and GPCR import.

Authors - Takuya Kobayashi
humanWB,IF

J Biol Chem

Overall architecture of the intraflagellar transport (IFT)-B complex containing Cluap1/IFT38 as an essential component of the IFT-B peripheral subcomplex.

Authors - Yohei Katoh
mouseIF

Cell Cycle

Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells.

Authors - Saul S Siller