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IFT57 Polyclonal antibody

IFT57 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, Canine and More (1)





Cat no : 11083-1-AP


DERP8, ESRRBL1, HIP1 interacting protein, HIPPI, IFT57, MHS4R2

Tested Applications

Positive WB detected inmouse retina tissue, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11083-1-AP targets IFT57 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse, rat, Chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT57 fusion protein Ag1566
Full Name intraflagellar transport 57 homolog (Chlamydomonas)
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC011899
Gene Symbol IFT57
Gene ID (NCBI) 55081
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFT57, also named as DERP8, ESRRBL1, HIPPI and MHS4R2, belongs to the IFT57 family. It is required for the formation of cilia. IFT57 plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. It is a component of IFT complex B which composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. In contrast to BBS proteins, IFT57 and IFT88 is found in almost every cilium.(PMID:22072986)


Product Specific Protocols
WB protocol for IFT57 antibody 11083-1-APDownload protocol
IHC protocol for IFT57 antibody 11083-1-APDownload protocol
IF protocol for IFT57 antibody 11083-1-APDownload protocol
IP protocol for IFT57 antibody 11083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Cell Biol

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.

Authors - Huijie Zhao

Nat Cell Biol

Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.

Authors - Malavika Raman

Dev Cell

The Intraflagellar Transport Protein IFT27 Promotes BBSome Exit from Cilia through the GTPase ARL6/BBS3.

Authors - Gerald M Liew

Nat Commun

IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.

Authors - Nicolas Taulet


Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Authors - Shichao Duan

Proc Natl Acad Sci U S A

Primary cilia signaling mediates intraocular pressure sensation.

Authors - Na Luo