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IGF1-Specific Polyclonal antibody
IGF1-Specific Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 20214-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 16 publications below |
IHC | See 4 publications below |
IF | See 1 publications below |
Product Information
20214-1-AP targets IGF1-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | insulin-like growth factor 1 (somatomedin C) |
Calculated Molecular Weight | 17 kDa |
Gene Symbol | IGF1 |
Gene ID (NCBI) | 3479 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGF1, also named as IBP1, MGF, IGF-IA and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but have a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. The antibody is specific to isoform IGF-1A.
Protocols
Product Specific Protocols | |
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IHC protocol for IGF1-Specific antibody 20214-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Gastroenterology Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1. | ||
J Hepatol Deficiency of insulin-like growth factor 1 attenuates aging-induced changes in hepatic function: Role of autophagy. | ||
Proc Natl Acad Sci U S A Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion. | ||
Int J Cancer Estrogen and insulin-like growth factor synergistically promote the development of lung adenocarcinoma in mice. | ||
Mol Neurobiol Intramuscular Delivery of scAAV9-hIGF1 Prolongs Survival in the hSOD1(G93A) ALS Mouse Model via Upregulation of D-Amino Acid Oxidase. | ||
Cancer Lett IFN-γ-mediated IRF1/miR-29b feedback loop suppresses colorectal cancer cell growth and metastasis by repressing IGF1. |