IGFBP2 Monoclonal antibody

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  WB analysis using 66644-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66644-1-Ig (IGFBP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 66644-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IGFBP2 antibody (66644-1-Ig, Clone: 2F10D1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

IGFBP2 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 66644-1-Ig

Clone No.2F10D1

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

IBP-2, IBP2, IBP 2, BP2, BP 2

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Tested Applications

• Positive WB detected in: A549 cells, T-27D cells, MCF-7 cells, PC-3 cells, NCCIT cells, 4T1 cells
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

66644-1-Ig targets IGFBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8421

  Product name: Recombinant human IGFBP2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 76-328 aa of BC004312

  Sequence: MPCAELVREPGCGCCSVCARLEGEACGVYTPRCGQGLRCYPHPGSELPLQALVMGEGTCEKRRDAEYGASPEQVADNGDDHSEGGLVENHVDSTMNMLGGGGSAGRKPLKSGMKELAVFREKVTEQHRQMGKGGKHHLGLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEARGVHTQRMQ

• Full Name: IGF binding protein 2
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC004312
• Gene Symbol: IGFBP2
• Gene ID (NCBI): 3485
• RRID: AB_2882002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P18065
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. The levels of IGFBP2 are elevated during progression of many human cancers.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
Human	IHC	Cancer Med; The preoperative neutrophil-to-lymphocyte ratio is not a marker of prostate cancer characteristics but is an independent predictor of biochemical recurrence in patients receiving radical prostatectomy.; Authors - Zhi Cao