IGFBP2 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 83283-3-RR (same clone as 83283-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83283-3-RR (IGFBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83283-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00322-2

  Sandwich ELISA standard curve of MP00322-2, human IGFBP2 Recombinant Matched Antibody Pair - PBS only. 83283-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0823. 83283-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00322-2

  Serum of eight individual healthy human donors was measured. The IGFBP2 concentration of detected samples was determined to be 109.5 ng/mL with a range of 47.4 - 199.1 ng/mL.

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  Sample test of MP00322-2

  Urine of eight individual healthy human donors was measured. The IGFBP2 concentration of detected samples was determined to be 6251.0 pg/mL with a range of 306.2 - 19423.8 pg/mL.

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  Sample test of MP00322-2

  MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM and 10% fetal bovine serum, 4 mM L-glutamine, 4500 mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human IGFBP2, and measured 3750.9 pg/mL. MDA-MB-453 human breast carcinoma cells (5 x 10^6 cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatants was removed, assayed for human IGFBP2, and measured 9611.6 pg/mL.

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  Affinity and Kinetic Characterization of 83283-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83283-3-RR against Human IGFBP2 were performed. The affinity constant is 1.85 nM.

IGFBP2 Uni-rAb^® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83283-3-PBS

Clone No.240227D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

IBP-2, IBP2, IBP 2, BP2, BP 2

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Product Information

83283-3-PBS targets IGFBP2 as part of a matched antibody pair:

MP00322-2: 83283-3-PBS capture and 83283-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: IGF binding protein 2
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: NM_000597.3
• Gene Symbol: IGFBP2
• Gene ID (NCBI): 3485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P18065
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kDa IGFBP protein with degraded fragments of 24 kDa, 19 kDa, and 15 kDa. The levels of IGFBP2 are elevated during the progression of many human cancers. IGFBP2 is expressed in invasive PAc, whereas its expression in HG-PIN is low. It can be as an Immunohistochemical Marker for Prostatic Adenocarcinoma.