Tested Applications
| Positive WB detected in | SH-SY5Y cells, T-47D cells, human plasma sample |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83283-3-RR targets IGFBP2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0823 Product name: Recombinant Human IGFBP-2 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 36-325 aa of NM_000597.3 Sequence: AEVLFRCPPCTPERLAACGPPPVAPPAAVAAVAGGARMPCAELVREPGCGCCSVCARLEGEACGVYTPRCGQGLRCYPHPGSELPLQALVMGEGTCEKRRDAEYGASPEQVADNGDDHSEGGLVENHVDSTMNMLGGGGSAGRKPLKSGMKELAVFREKVTEQHRQMGKGGKHHLGLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEARGVHTQRMQ Predict reactive species |
| Full Name | IGF binding protein 2 |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 33-36 kDa |
| GenBank Accession Number | NM_000597.3 |
| Gene Symbol | IGFBP2 |
| Gene ID (NCBI) | 3485 |
| RRID | AB_3670951 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P18065 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kDa IGFBP protein with degraded fragments of 24 kDa, 19 kDa, and 15 kDa. The levels of IGFBP2 are elevated during the progression of many human cancers. IGFBP2 is expressed in invasive PAc, whereas its expression in HG-PIN is low. It can be as an Immunohistochemical Marker for Prostatic Adenocarcinoma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IGFBP2 antibody 83283-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IGFBP2 Recombinant monoclonal antibody (240227D4), catalog number 83283-3-RR, has 1 total citation. The citation appears in In Vitro Cell Dev Biol Anim (2025), where the antibody was used by Western blot to study how growth hormone therapy promotes bone growth in idiopathic short stature children via the IGF-1 pathway and IGFBP2-mediated inhibition of THBS1. The research field encompasses pediatric endocrinology, bone biology, and growth factor signaling.
| Species | Application | Title |
|---|---|---|
In Vitro Cell Dev Biol Anim Growth hormone therapy promotes bone growth in idiopathic short stature children by activating the IGF-1 pathway via IGFBP2-mediated inhibition of THBS1.
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